Radioimmunoassay of serum estrogen.
نویسندگان
چکیده
Radioimmunoassay of serum estrogen (sum of estrone and estradiol-17f3) in 0.5 ml of human serum is described. After dilution of the serum, the estrogens are adsorbed on a Sephadex G-15 column in the presence of dilute HC1 and then eluted with benzene. After evaporation of the benzene eluate, the estrogens are dissolved in bovine serum albumin (5 g/. liter solution, at pH 8.0). An aliquot of this solution is used for the assay. Serum estrogen concentrations (mean ± SD) were 69 ± 23 pg/mI in 105 normal men and 199 ± 146 pg/mI in 26 fertile women. In 109 postmenopausal women, estrogen was undetectable in seven sera, but in the remaining 102 it was 52 ± 30 pg/mt of serum.
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عنوان ژورنال:
- Clinical chemistry
دوره 19 7 شماره
صفحات -
تاریخ انتشار 1973